| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Claesson, Emily E (16)
|
VATIDE |
23.57 |
24.24 |
0.67 |
| 2 |
Secrest, Jennifer L (19)
|
VATIDE |
23.73 |
24.64 |
0.91 |
| 3 |
Venema, Nicole E (19)
|
VATIDE |
22.84 |
25.03 |
2.19 |
| 4 |
McKee, Marin C (16)
|
VATIDE |
23.59 |
25.04 |
1.45 |
| 5 |
Durak, Anna J (20)
|
VATIDE |
24.38 |
25.25 |
0.87 |
| 6 |
Duffy, Kayleigh A (15)
|
VATIDE |
24.14 |
25.57 |
1.43 |
| 7 |
Hartley, Madison P (16)
|
VATIDE |
25.02 |
25.74 |
0.72 |
| 8 |
Dominick, Maggie M (17)
|
VATIDE |
23.58 |
26.35 |
2.77 |
| 9 |
Schooneman, Kim (16)
|
VATIDE |
25.05 |
26.51 |
1.46 |
| 10 |
Mosley, Kristilynn E (15)
|
VATIDE |
25.96 |
26.89 |
0.93 |
| 11 |
Benson, Alaiyah M (15)
|
VATIDE |
|
27.03 |
- |
| 12 |
Flora, Erin N (17)
|
VATIDE |
26.70 |
28.11 |
1.41 |
| 13 |
Worth, Spencer R (16)
|
VATIDE |
26.03 |
28.28 |
2.25 |
| 14 |
James, Kelsey M (16)
|
VATIDE |
25.03 |
28.30 |
3.27 |
| 15 |
Strecker, Morgan P (17)
|
VATIDE |
26.54 |
29.05 |
2.51 |
| 16 |
Neff, Megan M (17)
|
VATIDE |
|
29.78 |
- |
| 17 |
DeMieri, Emily N (17)
|
VATIDE |
27.65 |
29.94 |
2.29 |
| 18 |
Marks, Isabella G (16)
|
VATIDE |
|
31.38 |
- |
| 19 |
Moore, Tabitha K (17)
|
VATIDE |
29.76 |
31.99 |
2.23 |
| 20 |
Andersen, Victoria J (16)
|
VATIDE |
|
33.09 |
- |
| 21 |
Lucy, Raeleigh G (16)
|
VATIDE |
34.37 |
33.56 |
-0.81 |
| 22 |
Hudspeth, Lauren C (17)
|
VATIDE |
37.21 |
39.93 |
2.72 |